Fig. 2
Genomic CNV profiles across samples reveal metastatic waves. A Heatmap of copy number profile across all samples. Chromosomal coordinates are on the x-axis, and samples are plotted along the y-axis. Colors in the heatmap represent the log2 ratio of tumor copy number to normal copy number. Samples with similar CNV profiles are grouped together as G1, G2, G3, and G4. The shared CNVs in each group are highlighted in red boxes. Colored bars beside the sample names represent the host organs: lung(blue), bone(orange), brain(pink), breast(cyan), liver(green), kidney(grey), and pancreas(yellow). B An example of samples in the same group sharing complicated structural variants on chromosome 6, further confirming CNV-based grouping. Yellow and black lines are the two alleles of chromosome 6. A deletion occurred between 6p22.3 and 6p12.1 on one black allele in G2. A translocation occurred between chromosome 11 and one yellow allele of chromosome 6, designated as t(11;6)(p15.1;p11.2) (red and yellow line), and a translocation occurred between one black allele of chromosome 6 and chromosome 20, designated as t(6;20)(p22.3;p12.2) (black and green line), in G4. A translocation occurred between chromosome 4 and one black allele of chromosome 6, designated as t(4;6)(q32.1;q22.1) (blue and black line), in G3. C Copy number changes on chromosome 3 in different groups and inferred evolution. Yellow and black lines are two alleles of chromosome 3. G1, G2, G3, and G4 represent samples in each group. Each blue circle represents a cell population with certain chromosomal features inside