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Fig. 5 | Genome Medicine

Fig. 5

From: Functional screen of inflammatory bowel disease genes reveals key epithelial functions

Fig. 5

Impact of short- and long-term inhibitions of MAPKs on PIGR expression. The impact of short-term (24 h) and long-term (6 days) inhibitions of MAPKs on PIGR mRNA levels was evaluated by qPCR. A Exponentially growing HT-29-pLVX-Tet3G cell lines (n = 3) were either left untreated (no inhibitors; N.I.) or treated with chemical inhibitors specific for the different MAPKs (PD98059, ERKi; SB203580, p38i; SP600125, JNKi) at a concentration of 10 μM, and total RNA was isolated at two different timepoints (24 h and 6 days). Expression levels of PIGR were evaluated via qPCR; the qPCR results from the replicates were combined and mean expression values relative to samples without inhibitors (N.I.) are shown. B Exponentially growing HT-29-pLVX-Tet3G cell lines stably transduced with the TET3G-inducible expression plasmid for DUSP16 ORF (n = 3) were either left untreated (No doxycycline induction; No.Ind.) or stimulated with doxycycline at a concentration of 10 ng/ml to induce DUSP16. Cells were harvested at two different timepoints (24 h and 6 days) for total RNA isolation. Expression levels of PIGR were evaluated via qPCR; the qPCR results from the replicates were combined and mean expression values relative to samples without treatment with doxycycline (No.Ind.) are shown. All results are shown as geometric means with SEM. The values for independent replicates are shown (grey lozenges). As an indication, non-overlapping 3 datapoints from two conditions correspond to the strongest evidence against the hypothesis of no difference for a non-parametric test (P value = 0.1). Under hypothesis of lognormal distribution, the intervals as plotted correspond to 58% confidence interval for the median

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