Fig. 5

PLCG2 co-expression network analysis of microglial single-cell RNA-Seq data identified inflammatory response-related pathways. In the microglia single-cell RNA-Seq data, cells in the dorsolateral prefrontal cortex were isolated from 12 donors of both sexes. After quality control procedures, 15,021 cells were used for analysis. The UMAP plot shows the expression of PLCG2, and each dot represents a cell (a). The heatmap shows gene expression levels of the top 20 genes identified as co-expressed with PLCG2 using GENIE3 (b). The top ten GO terms from gene-set enrichment analysis of the top 20% of upregulated genes co-expressed with PLCG2 in human microglia in the disease condition (c). UMAP Uniform Manifold Approximation and Projection, MCI mild cognitive impairment, AD Alzheimer's disease