Fig. 5

Hybrid assemblies with Oxford Nanopore long-read and Illumina short-read sequencing data for isolates harboring Sig5.1-CP. Each colored oval represents an isolate harboring signature Sig5.1-CP. Specimen IDs and the years of isolation are indicated above each oval. The DNA molecules harbored by each of the isolates are represented by circles (or lines, since a linear DNA molecule was found in four isolates) with molecule sizes indicated in kb. The location of Sig5.1-CP is shown with yellow segments; a hallmark of this signature is the truncation of transposon Tn4401 by insertion sequence Tn5403. A schematic of the full 19-kb Sig5.1-CP is shown at the bottom of the figure. In this schematic, gene color corresponds to functional categorization: mobile genetic element (MGE) [yellow], carbapenem resistance [red], beta-lactam resistance [dark brown], and aminoglycoside resistance [light brown]