Fig. 1
From: A dynamic single cell-based framework for digital twins to prioritize disease genes and drug targets
Overview of the study. A Stimulation of PBMCs from SAR patients (red) and non-allergic controls (green) with allergen or diluent. B Key Th1/Th2 cytokines (IFN-γ, IL-4, IL-5, and IL-13) were measured in supernatants from SAR patients (yellow) and non-allergic controls (blue) over time. C (1) Time-series scRNA-seq of PBMC from [19]. (2) Construction of MNMs, showing predicted molecular interactions between cell types at each time point. (3) Ranking of URs by the number of cell types that each is predicted to regulate at different time points (see the “Materials and Methods” section and Fig. 6). (4) Prioritization of the top-ranking UR that regulates the greatest number of the cell types at the greatest number of time points—platelet-derived growth factor B (PDGFB). (5) Validation studies, in which two URs, IL4 and PDGFB, were blocked by specific antibodies