Fig. 4
Age-associated transcriptional differences of distinct memory-like NK cell subsets. A Volcano plots of the differentially expressed genes of NK2.1 (left), NK2.2 (middle), and NK2.3 cells (right) from comparisons between young and elderly individuals. Each dot represents a gene, with significantly upregulated genes (lnFC > 0.25, P < 10−3) in young and elderly individuals coloured blue and red, respectively. B Heatmap of the enriched GO terms among the DEGs detected for NK2.1, NK2.2, and NK2.3 cells (elderly vs. young). The colour indicates the -log10 (P-value) enrichment for each GO term. P-values were obtained with the Wilcoxon rank-sum tests. C Heatmap of the AUC scores predicted by SCENIC for expression regulation by transcription factors (TFs) in NK2.1, NK2.2, and NK2.3 cells from young and elderly individuals. D, E UMAP plots showing the AUC of the estimated regulon activity for IRF7, POLR2A, JUN, and JUNB (D) in NK2.1, NK2.2, and NK2.3 cells and the expression of these TFs (E). FC, fold change