Fig. 1

Overview of the study design. Left panel: Data from ChIP-Seq (conducted in neuronal tissues and cell lines) of 34 TFs from the ENCODE project were downloaded. After quality control, read mapping, and peak calling, binding motifs of the included TFs were derived. Right panel: SNPs in LD with the reported index SNPs were extracted (r² > 0.6) using genotype data for Europeans from the 1000 Genomes project. FIMO was used to evaluate motif occurrence in the sequences containing the SNPs of interest (sequences (±20 bp) flanking the SNPs of interest were used). A total of 16 functional candidate SNPs were selected, and a series of functional studies was performed to verify their regulatory functions