Fig. 4
Effect of individual protein changes on channel regulation by various modulators. A Boxes highlighted blue denote an increase in the measured parameter compared with matched WT controls where ↑P < 0.05, ↑↑P < 0.01, and ↑↑↑P < 0.001, and ↑↑↑↑P < 0.0001, determined using an unpaired Student’s t-test. Boxes highlighted gray denote a decrease in the measured parameter compared with WT, where ↓ P < 0.05, ↓↓P < 0.01, ↓↓↓P < 0.001, and ↓↓↓↓P < 0.0001. White boxes signify no change in effect from WT (←→P ≥ 0.05). Green boxes signify non-functional variants and mirror GFP-only transfected cells. B Current amplitude vs time. C Current amplitude vs Voltage. D Inhibition by acidic pH. E Inhibition by muscarine. ***P < 0.001 and ****P < 0.0001 for between group differences, determined using an unpaired Student’s t-test. Square or circle symbols represent individual data points for each condition and channel. Error bars represent 95% confidence intervals. pA, picoamp; pF, picofarad; VREV, reversal potential; ms, millisecond; mV, millivolt; M3, muscarinic receptor 3