Fig. 1

Single-cell transcriptome atlas of primary lung cancer. a Schematic diagram showing the experimental workflow of this study. Primary lung tumor tissues and matched normal tissues were collected from 19 primary lung cancer patients as well as 1 pulmonary chondroid patient who underwent surgery. After we got the freshly resected tumor tissues and matched normal tissues, we dissociated the tissues into single-cell suspension and rapidly picked the single cell into cell lysis buffer for single-cell RNA-seq analysis. b t-SNE plot of 7364 high-quality single cells showing the cell type identification, sample regions (LyM represents lymph node metastasis), patient information, and cell cluster information. c Expression levels of canonical cell type markers across 7364 single cells. d Heatmap showing the expression of specific cell type markers in six major cell types. e Proportions of identified six major cell types in normal tissues and tumor tissues separately across patients