Fig. 2
From: Molecular profiling of human non-small cell lung cancer by single-cell RNA-seq
Different lineage markers are co-expressed in the same individual tumor cells. a Heatmap showing the unsupervised hierarchical clustering of 3373 normal and tumor epithelial cells from 16 patients with the expression levels of NET, SCC, and ADC classical lineage markers. b Lineage score analysis further confirmed our cancer type identification. The ggtern plot displayed the cancer type score for each individual cell based on the expression levels of these cancer subtype lineage markers of SCC, ADC, and NET. “Highlight” cells represented cells from patient P12 with higher mixed features of ADC, SCC, and NEC. “Other” cells referred to the remaining cells except for ADC cells, NET cells, SCC cells, and “highlight” cells. c Barplot showing the component score for SCC, ADC, and NET cancer subtypes across every patient. d Multiplex fluorescent IHC staining of cells from patient P5 with p63 (TP63, SCC marker) and TTF1 (NKX2-1, ADC marker). Arrows indicated the double-positive cancer cells. Scale bar, 100 μm. e RNA in situ hybridization for TP63 and NKX2-1 in primary ADC tissues. Scale bar, 100 μm