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Fig. 4 | Genome Medicine

Fig. 4

From: Molecular profiling of human non-small cell lung cancer by single-cell RNA-seq

Fig. 4

AKR1B1 is essential for the proliferation of lung tumor cells. a Heatmap showing differentially expressed genes among normal epithelial cells, ADC-based single-lineage tumor cells, and combined four mixed-lineage tumor cells. b Box plot in the left shows the single-cell gene expression level of AKR1B1 in normal epithelial cells and five cancer cell subtypes. Box plot in right shows the single-cell gene expression levels of AKR1B1 in epithelial cells from normal tissues, tumor tissues, and LyM tissues. LyM represents lymph node metastasis. c Proliferation analysis of H2009 cells after AKR1B1 was knockdown with two different siRNAs. Compared with non-targeting control (NC), siAKR1B1-1 and siAKR1B1-2 significantly reduced the proliferation of H2009 cells. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. p values were determined by t-test. d Cell cycle analysis after H2009 cells were transfected with two different siRNAs of AKR1B1 for 48 h. Compared with NC, both siAKR1B1-1 and siAKR1B1-2 significantly decreased the cell fractions of S and G2/M phases of the cell cycle. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. p values were determined by t-test. e Cell apoptosis analysis after H2009 cells were treated with DMSO or 100 μM epalrestat for 48 h. Epalrestat treatment significantly promoted the apoptosis of H2009 cells. ****p < 0.001. p values were determined by t-test. f Photograph of tumors treated with sterile water or epalrestat 36 days after injection. These 11 tumors in the control group were derived from 7 mice, and these tumors in the treatment group were derived from 6 mice. Scale bar, 10 mm. g Quantitation of tumor volumes. The tumor volumes were calculated by the following formula: volume = length × (width)2 × 0.5. The maximum and minimum detected in each time point were removed. ***p < 0.001; ****p < 0.0001. p values were determined by t-test. h Quantitation of tumor weight from tumors in f. Epalrestat treatment significantly inhibited tumor growth. ***p < 0.001. p values were determined by t-test

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