Fig. 5

Actionability of the mutations in HLBCs. A Combined, double y-axis dot/barplot reporting the identified variants with OncoKB/ESCAT level of evidence (LoE). The left y-axis (barplot) reports the number of mutations per gene. The right y-axis shows the mean VAF level of these mutations (red dots). The bars were colored by the ESCAT LoE. B Lollipop plots for PIK3CA, ERBB2, and ESR1 genes, reporting the amino acid change, the mutation type, and the OncoKB LoE. Four variants of both PIK3CA (3 deletions, 1 SNV of the C2-domain) and ERBB2 (2 deletions, 1 SNV, and 1 non-sense mutation) showed no LoE. Gray dots define the mutations with unknown actionable significance. C Combined, double y-axis dot/barplot reporting the CNA genes with OncoKB oncogenic behavior in this cohort. The left y-axis (barplot) reports the number of patients with CNA per gene. Green bars depict the genes with CN gain, and blue bars depict those with CN loss. The right axis shows the mean fold change (FC) of these copy number alterations (CAN, red dots). Annotation at the bottom defines the available OncoKB/ESCAT LoE