Fig. 4
From: Single-cell transcriptomics reveals common epithelial response patterns in human acute kidney injury
PT AKI-enriched cell states reveal four distinct injury response patterns. A UMAP plot of subclustering of the PT with the anatomical PT segments S1-3 (PT-S1-3) and the AKI-associated cell states PT-New 1–4 (also depicted in Fig. 3A). Below the UMAP are a bar plot displaying the relative abundances of PT-New 1–4 with respect to all AKI PT cells and a trajectory analysis using partition-based graph abstraction (PAGA) highlighting diffusion pseudotime. Line widths of the connecting edges represent statistical connectivity between the nodes [56]. Healthy PT-S1-3 were summarized to healthy PT for this analysis. B Heatmap of selected marker genes for the identified PT cell subpopulations from marker gene analysis (Additional file 7: Table S6) and published markers for the anatomical segments PT-S1-3. C Plots display relative abundance of PT-New 1–4 as percentage of all PT cells. D Plot displays relative abundance of combined PT-New 1–4 as percentage of all PT cells. E Individual abundances of PT-New 1–4 for control and AKI individuals. P-value: * < 0.05, ** < 0.01, *** < 0.001; n.s., not significant. Control-PM, pooled samples (Control15 min, Control60 min, Control120 min) of post mortem non-AKI control individual