Fig. 5
From: Single-cell transcriptomics reveals common epithelial response patterns in human acute kidney injury
Multi-channel in situ hybridizations confirm the presence of the four “New” cell states in PTs. A In situ hybridizations for oxidative stress-related gene NQO1 (marker gene for PT-New 1), hypoxia-associated gene MYO5B (marker gene for PT-New 2) and canonical PT marker gene LRP2. Note that some MYO5B expression is observed in control samples as expected from the CPM values presented in B. B Feature plots highlighting the expression of NQO1 and MYO5B in PTs (compare to Fig. 4A) as well as box plots showing the expression of the respective gene in PTs in control versus AKI samples. C In situ hybridizations for inflammation response gene SERPINA1 (marker gene for PT-New 3), EMT-associated gene VCAM1 (marker gene for PT-New 4), and canonical PT marker gene LRP2. Scale bars as indicated. P-value: * < 0.05, ** < 0.01, *** < 0.001. CPM, counts per million