Fig. 6
From: Single-cell transcriptomics reveals common epithelial response patterns in human acute kidney injury
AKI-associated cell states within the thick ascending limb (TAL). A UMAP plot of TAL subclustering with the anatomical segments cTAL 1–3 and mTAL and the AKI-associated cell states TAL-New 1–4. Below the UMAP are a bar plot displaying the relative abundances of TAL-New 1–4 with respect to all AKI TAL cells and a trajectory analysis using partition-based graph abstraction (PAGA) highlighting diffusion pseudotime. Line widths of the connecting edges represent statistical connectivity between the nodes [56]. Healthy cTAL 1–3 and mTAL were summarized to healthy TAL for this analysis. B Heatmap of selected marker genes for the identified TAL cell subpopulations. C Plot displaying relative abundances of TAL-New 1–4 with respect to the individual’s TAL cells. D Relative abundances of combined TAL-New 1–4 cells with respect to the individual’s TAL cells. E Individual abundances of TAL-New 1–4 for control and AKI individuals. P-value: * < 0.05, ** < 0.01, *** < 0.001; n.s., not significant. Control-PM, pooled samples (Control15 min, Control60 min, Control120 min) of post mortem non-AKI control individual