Fig. 2

CXCR6 is expressed by CD8⁺ T cells that persist in the CNS after viral recovery. a, b Immunohistological analysis for CXCR6 and CD3 in the cortex (a) and hippocampus (b) of mock- or WNV-infected mice at 25 DPI, presented as microscopy. The left graph shows the percenter CD3⁺ area, the middle left graph shows the percent CXCR6⁺ area, the right middle graph shows the CD3⁺CXCR6⁺ area normalized to the total CD3⁺ area, indicative of co-localization, and the right graph shows the CD3⁺CXCR6⁺ area normalized to the total CXCR6⁺ area. Scale bars, 50 μm. c Gating strategy and quantification of the percentage of CD8⁺ T cells that are CXCR6⁺ at 7, 25, and 52 DPI in the cortex (left graph) and hippocampus (right graph). Cells were gated on CD45^high (Additional file 1: Fig. S8a) before gating on CD4⁺ and CD8⁺ T cells (left panel). CD8⁺ T cells were then gated on CXCR6 (middle panels). d Gating strategy and quantification of the percentage of CD103⁺ cells that are CXCR6⁺ at 7, 25, and 52 DPI in the cortex and hippocampus. e Gating strategy and quantification of the percentage of NS4B⁺ cells that are CXCR6⁺ at 7, 25, and 52 DPI in the cortex and hippocampus. f Gating strategy and quantification of the percentage of CD8⁺ T cells that are CXCR6⁺ at 7, 25, and 52 DPI in the blood. Cells were gated on CD45^high before gating on CD4⁺ and CD8⁺ T cells. CD8⁺ T cells were then gated on CXCR6. g Quantification of the percentage of CD8⁺ T cells that are CXCR6⁺ at 7, 25, and 52 DPI in the cervical lymph nodes. h Quantification of the percentage of CD8⁺ T cells that are CXCR6⁺ at 25 and 52 DPI in the meninges. Gating for CXCR6 based off Cxcr6 ^−/− mice (Additional file 1: Fig. S8b, c). Data represent the mean±s.e.m. and were analyzed by unpaired Student’s t-test. *P<0.05, **P<0.005, ***P < 0.001, ****P < 0.0001