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Fig. 5 | Genome Medicine

Fig. 5

From: Single-cell RNA transcriptome analysis of CNS immune cells reveals CXCL16/CXCR6 as maintenance factors for tissue-resident T cells that drive synapse elimination

Fig. 5

CXCR6 signaling potentiates gliosis in the recovered hippocampi of WNV-infected animals, contributing to synaptic elimination. a Representative immunostaining and quantification of IL-1β (green), GFAP (red), and DAPI (blue) in the CA3 region of the hippocampus of mock or WNV-infected WT or Cxcr6 −/− mice at 52 DPI. GFAP quantified by percent positive area, IL-1β quantified by percent positive area, followed by quantification of GFAP+IL-1β+ area, normalized to the total GFAP+ area. b Representative immunostaining and quantification of IBA1 (green), CD68 (red), and DAPI (blue) in the hippocampus of mock- or WNV-infected WT or Cxcr6 −/− animals at 52 DPI. IBA1+ quantified as percent positive area. c Representative immunostaining and quantification of C1qa in the hippocampus of mock- or WNV-infected WT or Cxcr6 −/− animals at 52 DPI showing staining for C1qa (green) and DAPI (blue). C1qa quantified as percent positive area. d Representative immunostaining and quantification of synapses in the CA3 region of the hippocampus in mock- or WNV-infected WT or Cxcr6 −/− animals at 52 DPI showing staining for synaptophysin (red) and DAPI (blue). Synaptophysin quantified by percent positive area. Scale bars, 50 μm (a–c) or 20 μm (d). Data represent the mean±s.e.m. and were analyzed by two-way ANOVA and corrected for multiple comparisons. *P<0.05, **P<0.005, ***P < 0.001

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