Skip to main content
Fig. 6 | Genome Medicine

Fig. 6

From: Single-cell RNA transcriptome analysis of CNS immune cells reveals CXCL16/CXCR6 as maintenance factors for tissue-resident T cells that drive synapse elimination

Fig. 6

CXCR6 on CD8+ T cells is necessary for the maintenance of TRM cells in the CNS after recovery from WNV infection. a Schematic showing the experimental design of CD8+ T cell adoptive transfer from WT and Cxcr6 –/– mice into Cd8 –/– mice. Twenty-four hours after adoptive transfer, mice were infected (i.c.) with 1×104 p.f.u. WNV-NS5-E218A and harvested at 52 DPI. b Flow cytometric analysis of the cortex and hippocampus at 52 DPI quantifying the percentage of CD45high cells that are CD8+ in WNV-infected mice that received WT or Cxcr6 –/– CD8+ T cells, followed by the percentage of CD8+ T cells that are CXCR6+ at 52 DPI in the mice that received WT CD8+ T cells. b Flow cytometric analysis of the hippocampus and cortex at 52 DPI quantifying the percentage of CD8+ T cells that are NS4B+ or CD103+ in WNV-infected mice that received WT or Cxcr6 –/– CD8+ T cells, followed by the total number of CD8+CD103+ T cells at 52 DPI in WNV-infected mice that received WT or Cxcr6 –/– CD8+ T cells. d Representative immunostaining and quantification at 52 DPI of IBA1 (green), CD68 (red), and DAPI (blue) in the hippocampus of WNV-infected mice that received WT or Cxcr6 –/– CD8+ T cells. IBA1+ quantified as percent positive area. e Representative immunostaining and quantification at 52 DPI of synapses in the CA3 region of the hippocampus of WNV-infected mice that received WT or Cxcr6 –/– CD8+ T cells showing staining for synaptophysin (red) and DAPI (blue). Synaptophysin quantified by percent positive area. Scale bars, 50 μm. Data represent the mean±s.e.m. and were analyzed by unpaired Student’s t-test. *P<0.05, **P<0.005, ***P < 0.001

Back to article page