Fig. 2

Kinetics of gHMW DNA degradation in blood fluids. A The kinetics of total and long DNA concentrations, as determined by qPCR, presented as a fraction of the initial concentration and DII (DNA integrity index) following gHMW DNA incubation up to 24 h at 37 °C in blood EDTA, plasma EDTA, and serum. Data are represented as mean ± SEM. B Size profiles of gHMW DNA samples before incubation in blood fluids (S0) and after incubation at 37 °C in plasma for 24 h (P24H) or in serum for 5 min (S5 min), 2 h (S2H), or 24 h (S24H) as determined by sWGS. C CirDNA fragment size profile of the thirteen healthy subjects, as determined by sWGS. Examination of gHMW DNA degradation and size analysis were performed and size profile using Y chromosome analysis