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Fig. 4 | Genome Medicine

Fig. 4

From: Neutrophil extracellular traps have auto-catabolic activity and produce mononucleosome-associated circulating DNA

Fig. 4

Kinetics of NET degradation in blood fluids. Kinetics of total and long (> 246 bp) nuclear cell-free DNA (cf-nDNA) concentration following the degradation of NET (supernatant of PMA-activated neutrophils) by incubation in serum/plasma up to 24 h: before incubation (S0) and after incubation at 37 °C in plasma for 24 h or in serum for 5 min/10 min/20 min/30 min/2 h/8 h/24 h. Data are represented as mean ± SD. B Analysis of cfDNA size profile by capillary electrophoresis (Fragment Analyzer) in the supernatant of PMA-stimulated (5 h) neutrophils (S0) and following its incubation in serum (S 5 min/S 10 min/S 20 min/S 30 min/S2H/S8H). C NET degradation in serum: the cfDNA size profiles of the supernatant of PMA-stimulated (5 h) neutrophils (PMA 5 h), and following incubation of this supernatant in serum at 37 °C for 10 min (S10), 2 h (S2H), and 24 h (S24H). D Analysis of sWGS data for assessment of the cfDNA fractions corresponding to mono-, di-, and trinucleosome-associated fragments during NET degradation. Examination of DNA degradation and of size profile were performed using Y chromosome

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