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Fig. 5 | Genome Medicine

Fig. 5

From: Neutrophil extracellular traps have auto-catabolic activity and produce mononucleosome-associated circulating DNA

Fig. 5

gHMW DNA degradation in presence of NE and MPO. A Quantification of total DNA by qPCR in cell lysate containing gHMW DNA before degradation (S0) and following gHMW DNA incubation at 37 °C for 2 or 8 h: in plasma or serum (control) and in plasma or serum in the presence of neutrophil elastase (+ NE), myeloperoxidase (+ MPO) or both (+ NE/MPO). B CfDNA size profiles for gHMW DNA samples before incubation in blood fluids (S0) and following 8 h of incubation at 37 °C in serum: control 8 h or in the presence of neutrophil elastase (+ NE), myeloperoxidase (+ MPO), or both (+ NE/MPO). C Analysis of cfDNA fractions corresponding to mono-, di-, and trinucleosome (Mono-N, Di-N, and Tri-N, respectively) associated fragments as determined by sWGS: cfDNA fractions in the course of gHMW DNA degradation in the presence of neutrophil elastase (+ NE), myeloperoxidase (+ MPO), or both (+ NE/MPO). Examination of gHMW DNA degradation and of size profile were performed using Y chromosome analysis

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