Fig. 4

SqCC Matreotypes have distinct cellular ecosystems. A–C Comparison of tumor purity (A, p<2.2E−16, Mann-Whitney U test), immunological enrichment (B, p=8.0E−10, Mann-Whitney U test), and stromal enrichment (C, p<2.2E−16, Mann-Whitney U test) in the ECM-High (green) compared with ECM-Low (blue) matreotypes identifies significant enrichment of both stromal and immune cells in ECM-High matreotype tumors. D–F Fibroblast cell types in the three major SqCC matreotypes ECM-Low (blue), ECM-High (green), and non-tumor (yellow) with fibroblasts from non-tumor tissue (D, ECM-Low vs ECM-High p=3.8E−10, ECM-Low vs Non-tumor p=7.3E−11, ECM-High vs Non-Tumor p=7.8E−11, Mann-Whitney U test), SqCC-associated fibroblasts (E, ECM-Low vs ECM-High p=0.85, ECM-Low vs Non-tumor p=0.59, ECM-High vs Non-Tumor p=0.66, Mann-Whitney U test) and tumor-associated myofibroblasts (F, ECM-Low vs ECM-High p=2.3E−22, ECM-Low vs Non-tumor p=0.036, ECM-High vs Non-Tumor p=1.8E−5, Mann-Whitney U test). G,H Epithelial cell types in the three major SqCC matreotypes ECM-Low (blue), ECM-High (green), and non-tumor (yellow) corresponding to type II pneumocytes (G, AT2, ECM-Low vs ECM-High p=0.0018, ECM-High vs non-Tumor p=3.1E−7, ECM-Low vs Non-tumor p=6.6E−5, Mann-Whitney U test) and secretory club cells (H, ECM-Low vs ECM-High p=0.00042, ECM-High vs non-Tumor p=1.8E−6, ECM-Low vs Non-tumor p=2.5E−8, Mann-Whitney U test). I The ECM-High matreotype is significantly depleted of normal endothelial cells (ECM-Low vs ECM-High p=0.034, ECM-High vs non-Tumor p=0.00038, ECM-Low vs Non-tumor p=0.013, Mann-Whitney U test). J–L Immunological cell types in the three major SqCC matreotypes ECM-Low (blue), ECM-High (green), and non-tumor (yellow) corresponding to regulatory T cells (J, ECM-Low vs ECM-High p=9.7E−10, ECM-High vs non-Tumor p=1.5E−7, ECM-Low vs Non-tumor p=0.0060, Mann-Whitney U test), B cells (K, ECM-Low vs ECM-High p=4.9E−6, ECM-High vs non-Tumor p=0.069, ECM-Low vs Non-tumor p=1, Mann-Whitney U test), and particularly the follicular subset of B cells (L, ECM-Low vs ECM-High p=0.0044, ECM-High vs non-Tumor p=3.7E−8, ECM-Low vs Non-tumor p=5.5E−5, Mann-Whitney U test).