Fig. 6

ECM-driven integrin signaling is associated with EMT and fibroblast activation in the ECM-High matreotype. A Ligand-receptor interaction results of differentially expressed core matrisomal genes and their cognate integrin receptors upregulated in ECM-High compared with ECM-Low matreotypes. Sector width is the aggregated log fold change of the ligand-receptor interaction strength comparing the ECM-High to ECM-Low matreotype. B Integrins are upregulated in the ECM-High compared with the ECM-Low matreotypes as indicated by the integrin receptor score. p=1.3E−23, Mann-Whitney U test. C Schematic of integrin-mediated signaling pathways active in the ECM-High matreotype. D–G Comparison of integrin-dependent signaling in the ECM-High (green) and ECM-Low (blue) matreotypes as indicated by the RPPA abundance of MEK1 pS217/S221 (D, p=1.6E−3), ERK pT202/204 (E, MAPK pT202/204, p=3.4E−4), Myosin IIA pS1943 (F, p=0.00027), and p21 (G, p=8.1E−8), Mann-Whitney U test. H–K Expression of genes SNAI1 (H, p=7.3E−12), ZEB1 (I, p=9.9E−15), ZEB2 (J, p=1.9E−21), and P21/CDKN1A (K, p=0.00014) in RNAseq data in the ECM-High (green) and ECM-Low (blue) matreotypes; Mann-Whitney U test. *** p<0.001, ****p<0.0001