Skip to main content
Fig. 3 | Genome Medicine

Fig. 3

From: Ganciclovir-induced mutations are present in a diverse spectrum of post-transplant malignancies

Fig. 3

Mycophenolate mofetil (MMF) treatment enhances ganciclovir (GCV)-associated mutagenesis. Fraction of CA > AA mutations in the H414 cell line (A), H023 organoid (B), and the HCT116 cell line (C) treated with vehicle (DMSO), MMF, ACV, ACV + MMF, GCV, or GCV + MMF. The concentration of MMF is 1 µM in all treatments. For H414 and HCT116 cells, the concentration of ACV and GCV is 100 µM. For H023, the concentration of GCV is 20 µM and 40 µM (2 ×). D GCVsig contribution score computed using sigfit for the mutational spectra of the treated cell lines and organoids in A–C. E Doubling time of H414 cells under different treatment conditions. GCV concentration was constant at 100 µM. The line indicates the mean of replicates. F Liquid chromatograph-tandem mass spectrometry-based quantification of GCV incorporation into genomic DNA under increasing MMF dosage in 100 µM GCV-treated H414 cells. The line indicates the mean of replicates

Back to article page