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Fig. 1 | Genome Medicine

Fig. 1

From: Cell type-specific changes identified by single-cell transcriptomics in Alzheimer’s disease

Fig. 1

A Current bulk profiling approaches that have generated large-scale data from post-mortem human brain tissue in the context of AD. Single-cell approaches have recently been scaled up to allow for a similar type of profiling, but have not reached the donor numbers interrogated using bulk techniques. B General workflow for single-cell approaches applied to human brain tissue. After isolation, individual cells or nuclei are encapsulated into wells or droplets, where lysis, reverse transcription, and amplification occurs, usually with some degree of pooling. Barcoded cellular/nucleus cDNA is then sequenced, and reads can be demultiplexed by cell/nucleus barcode and aligned to the transcriptome, ultimately generating expression profiles for each cell/nucleus. Panel A was created using the BioRender package

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