Fig. 1

scRNA-seq of BTBR ob/ob mouse kidneys with early DN. a Experimental scheme. b UMAP plot of annotated cell types. c Dot plot of defining marker genes for each cell type. d Total number of significant DEGs (nDEGs) in each cell type. Top enriched pathways in glomerular e and PT cell types f at 6 and 12 weeks. UMAP, uniform manifold approximation and projection; Ctrl, control; DN, diabetic nephropathy; Podo, podocyte; EC, endothelial cell; Mesan, mesangial cell; Int, interstitial cell; PT, proximal tubule; dLOH, descending limb of loop of Henle; aLOH, ascending limb of loop of Henle; DCT, distal convoluted tubule; CNT, connecting tubule; PC, collecting duct principal cell; IC-A, A-type collecting duct intercalated cell; IC-B, B-type collecting duct intercalated cell; Trans, transition cell; Imm, immune cell; Mitotic, mitotic cell