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Fig. 7 | Genome Medicine

Fig. 7

From: Single-cell transcriptomics reveals a mechanosensitive injury signaling pathway in early diabetic nephropathy

Fig. 7

Kidney ex vivo perfusion activates mechanosensitive signaling pathways. a Experimental scheme of mouse kidney ex vivo perfusion. b Heatmap showing the log2FC values of MRTF transcriptional target genes in perfused versus control glomeruli. c Top enriched pathways of MRTF transcriptional target genes. d Experimental scheme of pig kidney ex vivo perfusion. e UMAP plot of annotated cell types from snRNA-seq of perfused and control pig kidney tissue. g Immunofluorescence staining against MEIS1 in the pig kidney cortex. Nuclei were counterstained with DAPI. G, glomerulus. Dashed lines indicate the area of glomerulus. Scale bar: 100 μm. f Dot plot displaying defining marker genes for each cell type. STROMA, stromal cells. h Heatmap showing z scores of mechanosensitive transcriptional regulators estimated by IPA. i Dot plot displaying MRTF transcriptional target genes significantly changed in perfused kidney tissue. Nonsignificant regulation or genes are shown in gray g, h

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