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Fig. 2 | Genome Medicine

Fig. 2

From: TREM2 splice isoforms generate soluble TREM2 species that disrupt long-term potentiation

Fig. 2

Secretion of TREM2 isoforms. A Western blot analysis of TREM2 isoforms C-terminus (FLAG-tagged) and N-terminus (HA-tagged) in cell extracts (left panel) and media (right panel) of HEK-293T transfected with tagged TREM2 isoforms. The FLAG and HA antibodies were used to detect the C- and N-terminus, respectively. β-Actin and Ponceau staining were used as loading controls for cell extracts and media, respectively. B Immunocytochemistry in permeable and non-permeable conditions (presence and absence of detergents, respectively) was performed in HMC3 cells transfected with TREM2-tagged isoforms. The HA antibody was used to detect all TREM2 isoforms and DAPI to stain cell nuclei. C Scheme of the processing of TREM2 isoforms based on the transfection model

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