Table 2 Sequencing cost estimates at increasing matrix sizes using four commercial systems. Four commercial sequencers/flow cells used for sequencing cost estimates in Table 3
From: Combinatorial batching of DNA for ultralow-cost detection of pathogenic variants
System and flow cell | Read size (paired-end) | # of reads | Output (Gbp) | Price (USD) | Cost/Gbp (USD) | Cost/1X panel coverage (USD) |
|---|---|---|---|---|---|---|
NextSeq 500 Mid Output Kit v2 (300 cycles) | 150 × 2 | 400M | 120 | 5785 USD | 48.21 USD | 0.019 USD |
NovaSeq 6000 SP Reagent Kit v1.5 (300 cycles) | 150 × 2 | 800M | 240 | 5100 USD | 21.25 USD | 0.009 USD |
NovaSeq 6000 S2 Reagent Kit v1.5 (300 cycles) | 150 × 2 | 4B | 1200 | 12,150 USD | 10.13 USD | 0.004 USD |
NovaSeq 6000 S4 Reagent Kit v1.5 (300 cycles) | 150 × 2 | 10B | 3000 | 17,700 USD | 5.90 USD | 0.002 USD |