Fig. 3
From: spSeudoMap: cell type mapping of spatial transcriptomics using unmatched single-cell RNA-seq data
Predicting the spatial composition of region-specific neuron types in the mouse brain. A The proportion of the representative region-specific neuron types was estimated by spSeudoMap and mapped to the mouse brain. Overall, the 8 neuron types presented were predominantly localized to the corresponding anatomical locations. B Spatial spots were clustered based on their gene expression profiles, and the spot clusters were named after the anatomical locations. The predicted neuron fraction was highly distributed according to anatomical location. Ext_L25 to the mid-cortical layer, Ext_23 to the outer cortical layer, Ext_L56 to the inner cortical layer, Ext_Hpc_CA1, Ext_Hpc_CA3, and Ext_Hpc_DG1 to the hippocampus, Ext_Thal_1 to the thalamus, and Ext_Pir to the amygdala or piriform cortex area. Amy: amygdala, Amy_Pir: amygdala or piriform cortex, Cor_out: outer cortex, Cor_mid: mid cortex, Cor_in: inner cortex, CP: caudoputamen, EP: ependyma, Hippo: hippocampus, Hypo: hypothalamus, Men: meninges, Thal_lat: lateral thalamus, Thal_med: medial thalamus, and WM: white matter