Fig. 4

Heterogeneity of somatic copy number alterations in an untreated cancer. A Timeline of patient PAN113 showing sample collection and treatment. B Phylogenetic tree showing mutation clusters arising prior to, and after, systemic anti-cancer treatment. A putative driver mutation in CREBBP arises during treatment. C Heterogeneity of somatic copy number alterations (SCNAs). Events occur in parallel, potentially indicative of convergent evolution, or are heterogeneous between a metastatic sample and cfDNA extracted at the same time point. Ongoing heterogeneity is detected over time in subsequent cfDNA samples. D Copy number profiles of metastasis and three cfDNA samples of patient PAN113