Fig. 3

Uniform expression of OPC and COP markers in individual PM glioma cells. A–C Results of single-cell RNA-seq analysis of a representative PM glioma with 1p19q co-deletion from CGGA (sample ID: sc15). A t-SNE plot and cell numbers of the cell populations identified. B Heatmap of the top 200 most differentially expressed genes across the cell populations; lineage-specific hallmarks are shown. C Inferred chromosome CNVs in the OPC/COP-like cells with non-tumor cells as the control. D Concordant expression of SOX2 and early oligodendrocytic lineage markers but sporadic expression of astrocytic lineage markers in representative PM glioma samples with (MGH54, sc16 and sc15) or without 1p19q co-deletion (sc5, MGH43 and MGH45). O: oligodendroglioma grade 2, AO: anaplastic oligodendroglioma, OA: oligoastrocytoma grade 2, A:  astrocytoma grade 2, AA: anaplastic astrocytoma, O/C: OPC/COP-like cells, PLR: proliferating cells, APC: astrocyte precursor cells