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Fig. 2 | Genome Medicine

Fig. 2

From: Single-nucleus RNA sequencing of human pancreatic islets identifies novel gene sets and distinguishes β-cell subpopulations with dynamic transcriptome profiles

Fig. 2

Human islet cell type identification by projection strategy and mapping score assessment. A Azimuth pancreatic islet reference v1.0.1 (left) and scRNA-seq data projected on the Azimuth reference (right). B Cell type annotation prediction score displayed on dimensional reduction plot. C snRNA-seq data with only exon reads or with both exon and intron reads projected on the Azimuth reference (left), cell type projection score on dimensional reduction plot (right). D Prediction score in the snRNA-seq analysis with only exon reads or with both exon and intron reads in the projections in C. Statistical analysis using a Wilcoxon rank-sum test indicates a significant higher prediction score for the snRNA-seq data projection using exon plus intron reads. E Expression level and percentage of cells expressing canonical genes of Azimuth annotated endocrine cell types in the scRNA-seq data. F Expression level and percentage of cells expressing canonical genes of Azimuth annotated endocrine cell types in the snRNA-seq data. G Dimensional reduction plot for four representative canonical endocrine genes in the scRNA-seq data. H Dimensional reduction plot for four representative canonical endocrine genes in the snRNA-seq data

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