Fig. 6

Single-nucleus RNA-seq analysis of human islets in vivo in xenografts in immunosuppressed mice. A Human islet grafts processing and data analysis scheme. B In vivo snRNA-seq data obtained from four different human islet xenografts done with four different human islet preparations from adult human islets from healthy donors projected on Azimuth pancreatic islet reference and cluster annotation. C Dotplot depicting gene expression and percentage of cells expressing canonical gene markers (top) and newly identified gene markers for endocrine cells on Azimuth annotated in vivo data. D Projection of canonical genes and the four top newly found gene makers for endocrine cells from the in vivo snRNA-seq data on the dimensional reduction plot by Azimuth. E In vivo snRNA-seq data projected on internal reference with scRNA-seq and snRNA-seq of human islets in vitro integrated data and annotated. F Dotplot depicting the expression levels and percentage of cells expressing the canonical gene markers (top) and newly identified markers in the snRNA-seq of human islets in vitro (bottom) on internal reference annotated in vivo snRNA-seq data. G Projection of canonical genes and the four top gene makers for endocrine cells found in the snRNA-seq data from in vitro human islets of the in vivo snRNA-seq data on the dimensional reduction plot by internal reference