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Fig. 7 | Genome Medicine

Fig. 7

From: Single-nucleus RNA sequencing of human pancreatic islets identifies novel gene sets and distinguishes β-cell subpopulations with dynamic transcriptome profiles

Fig. 7

Human β-cell subtypes in vivo from the snRNA-seq data by projection on the in vitro internal reference. A Separated β-cell cluster from integrated main data identifying three cell sub-clusters using a Louvain resolution of 0.4. B INS expression pattern in the β-cell cluster in the in vivo snRNA-seq data on UMAP. C INS expression level on violin plots in the three β-cell subtypes in the in vivo snRNA-seq data compared with the in vitro snRNA-seq data. Notice the difference in expression in the β1 sub-cluster. D ZNF385D expression pattern in the β-cell sub-clusters in the in vivo snRNA-seq data on UMAP. E ZNF385D expression level on violin plots in the three β-cell subtypes in the in vivo snRNA-seq data compared with the in vitro snRNA-seq data. F HNRNPA2B1 expression pattern in the β-cell sub-clusters in the in vivo snRNA-seq data on UMAP. G HNRNPA2B1 expression level on violin plots in the three β-cell subtypes in the in vivo snRNA-seq data compared with the in vitro snRNA-seq data. H Proportion of the different β-cell subtypes in vivo and in vitro. Notice that in vivo, β1 cells become the majority of β-cells. Statistical analysis by Student’s t test indicates a significant increase in vivo of β1 cells while β2 and β3 cell subtypes are reduced

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