Fig. 3

Distinct transcriptomic features and immunogenic phenotype between primary and metastatic lesions in FH-RCC. A Enrichment analysis of paired upregulated genes between metastatic lesions and primary lesions. B Paired ladder box plots depict the changes of immune score in patient-matched cases. Y-axis represents ssGSEA Z-score. P-value was determined by paired t test. C Paired ladder box plots depict the changes of tumor proliferation rate in patient-matched cases. Y-axis represents ssGSEA Z-score. P-value was determined by paired t test. D Paired ladder box plots depict the changes of cell cycle in patient-matched cases. Y-axis represents ssGSEA Z-score. P-value was determined by paired t test. E Heatmap depicts the conserved pan-cancer microenvironment signature analysis in paired normal, primary, and metastatic lesions. ssGSEA Z-score are used. F Paired ladder box plots depict the changes of Th1 cells, T cells, effector cells, effector cells traffic, co-activation molecules, and antitumor cytokines in patient-matched cases. Y-axis represents ssGSEA Z-score. P-value was determined by paired t test