Fig. 4

Immune evasion mechanisms of metastatic FH-RCC. A Volcano plot of DEGs in metastatic lesions compared with paired primary lesions of FH-RCC. B Expression of chemokine ligand family (CCL21, CCL19, CCL2, CCL3, CCL4L2, CCL18) in metastatic lesions, compared to paired primary lesions. Y-axis represents TPM of gene expression. P-value was determined by Wilcoxon signed rank test. C Correlation analysis of chemokine ligand family molecules (TPM) and immune microenvironment signatures(ssGSEA Z-score). D Paired ladder box plots depict the change of myeloid-derived suppressor cell-related signatures in patient-matched cases. Y-axis represents ssGSEA Z-score. P-value was determined by Spearman correlation analysis. E Representative pictures of multiple immunofluorescence staining demonstrated RNA signature-based difference of CD68 + /CD163 + TAMs between primary and metastatic lesions, and quantitative analysis of CD8 + , CD68 + /CD163 + cells in cases FH42 and FH48. P-value was determined by t test. Scale bar: 100 µm