Fig. 5

The expression of immune checkpoints in metastatic FH-RCC. A Paired ladder box plots depict the checkpoint molecule signatures (measured by ssGSEA analysis) change in patient-matched cases. Y-axis represents ssGSEA Z-score. P-value was determined by paired t test. B Paired ladder box plots depict the changes of CD274, BTLA, and TIGIT in patient-matched cases. Y-axis represents TPM of genes. P-value was determined by Wilcoxon signed rank test. C Expression and quantitative analysis of PD-L1 by IHC in metastatic and paired primary lesions. Scale bar: 100 µm. D Expressions of CD8 and TIGIT were quantitatively analyzed at the protein level by multiple immunofluorescence staining in patient-matched cases. P-value was determined by t test. Scale bar: 100 µm