Fig. 6

Astrocytes are localised to the mixed tumour-microenvironment interface. a Palbociclib A and Control C shown with annotated mouse cell types depicting the localisation of astrocytes to the interface region. Spot colours indicate cell types. Red boxes indicate the region of interest enlarged on right. b The equivalent to a but with expression of the astrocyte marker genes Gfap. The gradient colour reflects log cpm (count per million). A strong concordance between astrocyte localisation and Gfap expression is observed, with both localised at the interface region. b The reactive astrocytes (Gfap) located in the tumour–mouse interface. c Positive proliferating cells (Ki67) are located throughout the tumour in untreated tumours, but localised to the tumour–mouse interface in drug-treated PDOX in close proximity with astrocytes, as identified by GFAP staining. d Target RNA molecule expression of astrocytes (Gfap) and proliferative tumour cells (MKI67) at a single-cell level using RNAscope