A ‘fit’ microbiota to potentiate cancer immunotherapy

Cancer immunotherapy is very effective and leads to a long-term response in certain patients. Yet, the variability observed in this response indicates that additional factors related to the host must influence the activity of the treatments. Recent research suggests that the microbiota might play an important part in this variability.

These inhibitors reactivate T cells to 'resurrect' them from an ineffective state that does not allow them to respond to antigens. However, we do not know how T cells are reactivated and what the characteristics of the patients who respond are.

Learning from mouse models
Vetizou et al. [1] and Sivan et al. [2] show that the efficacy of ICI treatment is dependent on the host microbiota. Indeed, in mice reared under germ-free conditions or treated with antibiotics the ICIs lost their therapeutic efficacy. In both studies, the authors found that, in the presence of the microbiota, host antigen-presenting cells activate interferon (IFN)γ-producing T cells, which are enriched during ICI treatment. It is amazing that the microbiota contributes to immune cell activation at distant sites and in particular tumor sites. The researchers excluded the possibility that these effects occur through systemic dissemination of the microbiota. This observation raises the question of whether microbial metabolites disseminate systemically and reach tumor compartments or whether these metabolites act on peripheral lymphoid organs.
In the study by Vetizou et al. [1], the researchers identified several Bacteroides species, including Bacteroides fragilis, and polysaccharide A produced by this bacterium as capable of promoting the maturation of intratumoral dendritic cells and inducing type 1 helper T cells in tumor-draining lymph nodes. Sivan et al. found that wild-type C57BL/6 mice from two different providers, the Jackson Laboratory (Jax) and Taconic (Tac), exhibited significant differences in the rate of melanoma growth, with tumors growing faster in Tac mice. The same trend was observed when mice were treated with anti-PD-L1, with a better response observed in Jax mice than in Tac mice. The authors compared the microbiotas of the mice housed in the two animal facilities and correlated their components with the amount of activated antigen-presenting cells in the tumor microenvironment. Only the levels of Bifidobacterium breve, Bifidobacterium longum and Bifidobacterium adolescentis were positively associated with the amount of antigen-presenting cells in tumors. Interestingly, administration of a mixture of B. breve and B. longum to Tac mice resulted in improved tumor control and increased IFNγ levels in tumor-draining lymph nodes and spleen.

Microbial diversity and therapy outcome
These results indicate that having a 'fit' microbiota helps the immune system to perform effective immune surveillance. They also raise the questions of what a 'fit' microbiota is and how we can intervene to provide the best microbiota to patients. As the diversity of the microbiota is in part genetically determined [8], are some individuals predisposed to have a less effective microbiota, is the microbiota shaped during tumor development, or are both of these statements true?
Interestingly, Vetizou et al. [1] show that administration of B. fragilis or Bacteroides thetaiotamicron to wildtype mice can alter the activity of anti-CTLA4 in vivo, and also reduce the inflammatory response initiated by this antibody in the intestine. These findings indicate that the right bacterial combination can both potentiate the activity of ICIs and provide protection from the adverse effects of therapy, thus 'uncoupling' efficacy and toxicity of the antibody. The researchers also found that administration of anti-CTLA4 modifies the microbiota composition and increases the levels of the strains that seem to have a beneficial antitumor effect. These findings were paralleled by observations in patients with metastatic melanoma who were treated with anti-CTLA4. The researchers found that patients could be divided into three groups according to their microbiota (enterotypes) and that two enterotypes were associated with a better outcome than the other enterotype. The 'good' enterotypes were enriched in some, but not other, Bacteroides species that mediate the therapeutic effect of the drug, whereas the 'bad' enterotype still had quite a high number of B. fragilis, which potentiated the efficacy of anti-CTLA4 in mice. These findings suggest that either this species is effective only in the right microbial context or that some individuals select strains of B. fragilis that are more beneficial than others despite belonging to the same species.
It is obvious that these studies have huge therapeutic implications, but they also raise important issues. Can we improve an individual's microbiota to achieve maximal therapeutic efficacy of immunotherapy? Is it sufficient to administer one species, such as B. fragilis or B. breve, or should we give a mixture of microorganisms, or even perform fecal transplantation of the microbiota? If two therapeutic options are available, should we select or exclude them according to the enterotype of the patient? As chemotherapy and cytokine-based immunotherapy also rely on the microbiota for their efficacy [9,10], are there different enterotypes that mediate the response to different therapeutic agents or are there enterotypes that favor any possible therapy regardless of whether it is chemotherapy, immunotherapy or targeted therapy?
One thing is clear from these studies: the composition of our microbiota should be considered in future clinical studies aimed at assessing the therapeutic efficacy of new anticancer agents.