Lower IgG somatic hypermutation rates during acute dengue virus infection is compatible with a germinal center-independent B cell response
- Elizabeth Ernestina Godoy-Lozano1,
- Juan Téllez-Sosa†1,
- Gilberto Sánchez-González1,
- Hugo Sámano-Sánchez1,
- Andrés Aguilar-Salgado1,
- Aarón Salinas-Rodríguez2,
- Bernardo Cortina-Ceballos1,
- Héctor Vivanco-Cid3,
- Karina Hernández-Flores3,
- Jennifer M. Pfaff4,
- Kristen M. Kahle4,
- Benjamin J. Doranz4,
- Rosa Elena Gómez-Barreto1,
- Humberto Valdovinos-Torres1,
- Irma López-Martínez5,
- Mario H. Rodriguez1 and
- Jesús Martínez-Barnetche†1Email author
© Godoy-Lozano et al. 2016
Received: 11 October 2015
Accepted: 3 February 2016
Published: 25 February 2016
The study of human B cell response to dengue virus (DENV) infection is critical to understand serotype-specific protection and the cross-reactive sub-neutralizing response. Whereas the first is beneficial and thus represents the ultimate goal of vaccination, the latter has been implicated in the development of severe disease, which occurs in a small, albeit significant, fraction of secondary DENV infections. Both primary and secondary infections are associated with the production of poly-reactive and cross-reactive IgG antibodies.
To gain insight into the effect of DENV infection on the B cell repertoire, we used VH region high-throughput cDNA sequencing of the peripheral blood IgG B cell compartment of 19 individuals during the acute phase of infection. For 11 individuals, a second sample obtained 6 months later was analyzed for comparison. Probabilities of sequencing antibody secreting cells or memory B cells were estimated using second-order Monte Carlo simulation.
We found that in acute disease there is an increase in IgG B cell diversity and changes in the relative use of segments IGHV1-2, IGHV1-18, and IGHV1-69. Somewhat unexpectedly, an overall low proportion of somatic hypermutated antibody genes was observed during the acute phase plasmablasts, particularly in secondary infections and those cases with more severe disease.
Our data are consistent with an innate-like antiviral recognition system mediated by B cells using defined germ-line coded B cell receptors, which could provide a rapid germinal center-independent antibody response during the early phase of infection. A model describing concurrent T-dependent and T-independent B cell responses in the context of DENV infection is proposed, which incorporates the selection of B cells using hypomutated IGHV segments and their potential role in poly/cross-reactivity. Its formal demonstration could lead to a definition of its potential implication in antibody-dependent enhancement, and may contribute to rational vaccine development efforts.
Dengue, the most prevalent mosquito borne-viral disease in humans, is caused by four closely related serotypes of dengue virus (DENV) of the Flaviviridae family. The infection can be asymptomatic, produce a mild self-limited febrile disease lasting up to 10 days, or result in a severe disease with hemostatic and hemodynamic dysfunction [1, 2]. According to recent estimates, 390 million dengue infections occur each year, of which only 96 million reach the public health surveillance system .
DENV primarily infects dendritic cells and monocytes [4–6]. During the early stages of infection, a rapid, strong innate immune response is elicited, resulting in a systemic inflammatory immune response that explains the majority of symptoms of uncomplicated illness. Shortly after, an adaptive immune response manifests as the production of low-affinity IgM anti-DENV antibodies, and, later, high-affinity anti-DENV IgG-neutralizing antibodies that confer long-term protection to the infecting serotype (homotypic protection). However, primary infections are also associated with the production of low-affinity, non-neutralizing or sub-neutralizing cross-reactive antibodies against multiple DENV-serotypes that only confer partial short-term protection .
An almost paradigmatic feature of dengue infection is the epidemiological observation that severe illness is associated with a previous infection with a different DENV serotype . Non-neutralizing cross-reactive antibodies induced during primary infections have been implicated in potentiating viral replication, systemic immunopathology, and severe disease by FcγR-mediated antibody-dependent enhancement (ADE), offering a plausible explanation of the increased disease severity after multiple reinfections .
Antibody production is the result of B cell clonal selection, expansion, and differentiation into antibody-secreting plasma cells. In the case of protein antigens, antigen-specific B cells are clonally selected and form germinal centers (GC) depending on T cell help. Within the GC, selected B cells undergo somatic hypermutation (SHM) and class switch recombination (CSR) [10, 11], which are two mechanistically coupled processes resulting in high affinity IgG-switched memory B (mB) cells and antibody-secreting plasma cells . However, it has become increasingly clear that B cell memory is heterogeneous in terms of their origin and function . In the context of DENV infection, the generation of long-lived anti-DENV IgG+ memory cells selected by a primary infection has been implicated in the predominance of neutralizing antibodies against the primary infecting serotype over neutralizing antibodies specific for a different serotype causing a secondary infection . Presumably, this so-called original antigenic sin could also favor ADE by outcompeting secondary serotype anti-DENV high-affinity B cell clones. Consistently, poly-reactive and serotype cross-reactive IgGs are present in high titers in early primary and secondary DENV infections [15–19]. Thus, understanding B cell responses to DENV infection as well as antibody-mediated immune protection and enhancement requires the integrative analysis of the clonal selection process within the actual conceptual framework of pathway heterogeneity of effector B cell differentiation .
Given the central role of antibodies and their affinity in the protection against DENV and their implication in severe disease, we hypothesized that the B cell clonal selection process and diversification may be different between primary and secondary dengue infections, and between DENV infections without clinical warning signs (DWS−) and dengue infections with clinical warning signs (DWS+). To identify these differences, we used high-throughput sequencing (HTS) of peripheral blood IgG antibody repertoires derived from cDNA (reviewed in [20, 21]) to compare clonal diversity, IGHV usage, and SHM rates between patients with acute DWS− and DWS+ and their corresponding post-convalescent blood samples (6 months after).
We found differential changes in IGHV1-2, IGHV1-18, and IGHV1-69 clonal usage frequency and transcription. Paradoxically, we observed overall lower SHM rates during acute illness, particularly in DWS+ and in lymphocytes using IGHV1-2, suggesting their implication in cross-reactive low-affinity IgG antibodies. Our results also suggest that in humans infected with DENV, in addition to a classical GC pathway, an alternative GC-independent effector B cell differentiation pathway may take place, in which CSR to IgG is decoupled from SHM.
This study was conducted according to the principles expressed in the Declaration of Helsinki. The study was approved by the Research, Ethics and Biosafety Committees of the Instituto Nacional de Salud Pública (CI:1023/1100), Universidad Veracruzana, Integral Molecular and Instituto de Diagnóstico y Referencia Epidemiológicos (InDRE). Written informed consent was obtained from all participants.
Patients, donors, and samples
During dengue season 2010 and 2011, 19 adult patients with clinical and laboratory confirmed DENV infection living in Veracruz, a DENV endemic zone in Mexico , were enrolled after providing written informed consent. Patients were classified as DWS+ if they required hospitalization, had hematocrit > 40, a platelet count < 100 × 103, and at least one of the following signs: abdominal pain or tenderness, persistent vomiting, clinical fluid accumulation, mucosal bleeding, lethargy, restlessness, or liver enlargement (>2 cm). Patients donated two peripheral blood samples to provide total RNA and serum: one during the febrile stage (acute sample) and the other 6 months after recovery (post-convalescent sample), coinciding with the low transmission season to minimize the possibility of asymptomatic reinfection. For some data analyses, an additional control group of 10 healthy volunteers enrolled in an influenza vaccination study , was included. Peripheral blood was collected to obtain serum and total RNA. For RNA isolation, 2.5 ml of peripheral blood in PaxGene RNA tubes (QIAGEN GmbH, Hilden, Germany) was used according to manufacturer’s instructions and stored at −70 °C until use.
DENV serological diagnosis of primary and secondary cases
All sera were tested with reference anti-NS1 enzyme-linked immunosorbent assay (ELISA) with the Platelia Dengue NS1 Ag Test (Bio-Rad, Marnes-la-Coquette, France) and Dengue IgM (Panbio®, Sinnamon Park, QLD, Australia). To discriminate between primary and secondary infections, we used the IgG Capture ELISA (Panbio®). Primary cases were defined as a positive reverse-transcriptase polymerase chain reaction (RT-PCR), NS1, and/or anti-IgM ELISA and negative anti-IgG ELISA. Secondary cases were defined as positive anti-IgG ELISA and NS1/or RT-PCR, regardless of the results of the anti-IgM ELISA.
Dengue virus genotyping
Viral RNA was isolated from the sera during the acute febrile phase (QIAamp Viral RNA mini kit, Qiagen) and used to determinate DENV serotype by quantitative RT-PCR according to the protocol of InDRE, Mexico , and the Official Mexican Norm: NOM-032-SSA-2002 .
DENV reporter virus particles neutralization assay
DENV reporter virus particles (RVPs) for the four serotypes  were pre-incubated with an equal volume of all serially diluted acute sera (1:10 to 1:10,240; all dilutions were pre-incubated with RVPs) in complete Dulbecco’s Modified Eagle Medium for 1 h at room temperature with slow agitation. Following incubation, BHK DC-SIGN cells were added to each well at a density of 30,000 cells per well, followed by incubation at 37 °C in 5 % CO2 for 72 h. Cells were subsequently lysed and analyzed for luciferase luminescent reporter expression. The percent infection for each concentration of serum was calculated using Prism software 5.0 and raw data expressed as percent infection versus log10 of the reciprocal serum dilution. A sigmoidal dose–response curve with a variable slope was applied to determine the titer of antibody that achieved a 50 % reduction in infection (50 % neutralization titer, NT50). Maximum infection was determined using a no serum control. An NT50 ≥ 1:50 was defined as a positive neutralization test.
VH libraries and high throughput cDNA sequencing
The RNA concentration and integrity was analyzed through capillary electrophoresis in an Agilent 2100 BioAnalyzer, with the RNA 6000 Pico kit. cDNA was generated for the VH region of IgG through 5′ rapid amplification of cDNA ends (RACE)-PCR, using a protocol modified from the SMART RACE cDNA Amplification Kit (Clontech Laboratories, Inc.). The forward primer (FpAmpTA) was a modification from the UPM primer to which we added to the 5′ end an A adapter from the platform GS FLX Titanium 454-Roche. The reverse primer TBIgGHu [5′-(454adaptorB)CTA TGC GCC TTG CCA GCC CGC (454key) TCAG(IGHG)ACC GAT GGG CCC TTG GTG-3′] primes in Exon I of the IGH G genes and has the B adapter for the 454-Roche sequencing . We analyzed the 500–600 bp 5′RACE-PCR products with 1.5 % agarose gel electrophoresis and purified them with a MiniElute PCR purification kit (Qiagen). Their concentration and integrity was analyzed through capillary electrophoresis in a 2100 BioAnalyzer using the High Sensitivity DNA 2100 LabChip (Agilent Technologies).
We used 100 ng of each library for the emulsion PCR (GS emPCR Kit, 454-Roche). HTS was performed using Genome Sequencer FLX Titanium System 454-Roche with the GS LR70 Sequencing Kit according to the manufacturer’s instructions. This platform generates an average read length of 450–500 bp. We performed the sequencing with the B adapter (3′ → 5′) so that the complementarity determining region heavy 3 (CDRH3) region was proximal and the 5′ UTR was the sequencing primer, allowing higher sequencing quality in the majority of the IGHV coding region. Raw sequencing files are available in NCBI-SRA: BioProject ID: PRJNA302665; accession number: SAMN04277236-65.
Estimation of probabilities of sampling either IgG+ antibody-secreting cells or memory B cells
To overcome the limitation of working with unsorted IgG+ B cell subpopulations [antibody-secreting cells (ASC) or mB], we designed a computational protocol consisting of a second-order Monte Carlo simulation to estimate the probability of picking a progressive number of clonally related sequencing reads belonging to either population for each cell sampled, during the acute disease and post-convalescence . The model accounts for individual variation over a gradient of relative proportions of ASC and mB cells, as well as cellular variation in the relative Ig transcription levels in both subpopulations. Briefly, the protocol calculates the probability of sampling Ig transcripts from either subpopulation by random sampling distributions corresponding to the relative amount of either subpopulation in a blood sample, as well as the relative amount of Ig transcripts per cell. The process is calculated in 500 individuals, for a given mB cell to ASC ratio that begins with 1 % of ASCs in post-convalescent individuals and ends with 1 % of mB cells (Additional file 1). In the simulation, an average of 1000 IgG+ B cells having a normal distribution and 5 % variance were randomly sampled. The Ig expression in mB cells has a normal distribution with a mean of 100 arbitrary units (au) and 5 % variance, and the Ig expression in ASCs follows a gamma distribution with central value of 1200 au (12-fold increase relative to an mB cell), a minimal value of 300, and a maximal value of 10,000 au  (Additional file 1).
Pre-processing and repertoire reconstruction
We have developed a software (pipeline) named ImmunediveRsity for the analysis of the repertoire sequencing (Rep-Seq) data . ImmunediveRsity is written in R language  and automates Ig sequencing analysis from pre-processing, error correction and quality filtering, V(D)J segment assignment, CDRH3-based sequence clustering for heavy chain clonotypes, and their further clustering into heavy chain lineages as a result of clonotype diversification by SHM (referred hereafter as clonotypes and lineages, respectively. Additional file 2). Raw sequences with an average ≥ Q28 value and reads ≥250 bp passed the quality filter. In order to exclude non-VH sequences, ImmunediveRsity assigns IGHV and IGHJ segment use to each read using IgBLAST . A clonotype is composed by reads that share the same V and J segment and their CDRH3 has the same length and is 97 % identical . To discard a possible effect of a CDRH3 clustering threshold on SHM, repertoire data are also reconstructed at a 92 % identity threshold. Reads belonging to a clonotype are further clustered along the whole coding region, excluding the signal peptide, so that the lineage is the consensus of reads sharing 99.5 % identity (Additional file 2). For the analysis of IGHV usage, collapsing sequences according to a common clonal origin and to a particular lineage allows the frequency to be expressed according to total clonotypes or lineages, regardless of Ig transcription levels. Thus, a given clonotype composed of 80 % of the sequencing reads has an equal clonotype frequency to that of a clonotype composed of 0.1 % of the sequenced reads. The same applies for lineages. For ImmunediveRsity, a lineage is an approximation of a single B cell, although it is possible to underestimate the true B cell numbers (for example, when two B cells from the same clonotype are identical or the proportion of SHM is below the clustering threshold of 99.5 % identity). ImmunediveRsity output files for each sequenced library can be found at http://220.127.116.11:8080/dengue-project-2015/.
Estimation of sample B cell diversity
ImmunediveRsity calculates clonotype and lineage entropy values (Shannon Index) [33, 34] and performs a rarefaction analysis  as indirect measures of lymphocyte diversity. The number of reads per clonotype and lineage obtained for each sample (acute or post-convalescent phase) was used to calculate the Shannon Index. Rarefaction curves were calculated with the number of clonotypes in growing subsamples of 1000 reads.
IGHV segment overrepresentation analysis
We used three approaches to identify overrepresented IGHV segments during acute DENV infection. The first approach aimed to reflect IGHV use based on the estimation of IGHV segment relative transcription levels, regardless of clonotype and lineage composition, and was calculated based on the proportion of reads for each IGHV family and segments normalized to the total number of reads per library (raw IGHV usage). When both acute and post-convalescent samples were available, the proportion of reads for each segment during the acute (A) phase was subtracted from its corresponding value during the post-convalescent (Pc) phase (ΔA–Pc). The second and third approaches aimed to estimate IGHV usage per clonotype or lineage, respectively, where the number of clonotypes or lineages using a particular IGHV segment was expressed as the proportion of all clonotypes or lineages in the corresponding library using a particular IGHV segment. Similar to the first approach, changes of IGHV usage are expressed as the difference of the acute phase frequency minus its corresponding post-convalescent frequency (ΔA − Pc). Statistical evaluation was done with a two-way analysis of variance (ANOVA) with Bonferroni correction for multiple testing using Graph Pad Prism v5.0. Differences were considered statistically significant if p < 0.05.
VH mutation analysis
The numbers of non-synonymous and synonymous mutations were obtained with IMGT/HighVQuest  for each lineage consensus. To compare the proportion of mutations, only productive lineages were used for random sub-sampling (1280 lineages per library, which corresponded to the library with the least number of lineages). The proportion of mutations (pM-VH), proportion of non-synonymous mutations, and the proportion of synonymous mutations were calculated as the percentage of total mutations in the VH region, excluding the CDRH3, divided by its length. To avoid non-independence effects from lineages derived from large clonotypes, SHM was also calculated in the largest lineage per clonotype from 250 randomly sampled clonotypes. To identify differences in the proportion of mutations per IGHV segment, the mean global mutation proportion was subtracted from each individual IGHV mean mutation proportion. The difference was used for unsupervised hierarchical clustering according to IGHV segment using an uncentered correlation metric for clustering with CLUSTER 3.0 . We performed multivariate comparisons among control, DWS− A, DWS+ A, DWS− Pc, and DWS+ Pc consensus of lineages based on different metrics including the mean of the proportion of mutations (non-synonymous and synonymous), the mean frequency of lineages, and CDRH3 length. A multilevel principal component analysis  was applied in a sample of 1280 randomly chosen lineages for each individual, and graphically visualized using biplot graph, which is a graphical representation of principal component 1 (PC1) versus principal component 2 (PC2), which are selected by the proportion of explained variance (that is, accounting for as much of the variability in the data as possible). This analysis was conducted using R software . Non-parametric analyses (Kruskal–Wallis test) with Dunn’s correction for multiple testing were performed for comparisons among the different groups with R software . Differences were considered statistically significant if p < 0.05.
CDRH3 convergence analysis
CDRH3 convergent signatures have been described in acute DENV infection . We used two approaches to identify CDRH3 convergent signatures: the first was based on searching for the previously described signatures in our VH clonotype databases in acute infection and post-convalescence. The second was based on de novo identification of shared CDRH3 within our datasets. For both approaches, the R function Find_CDR3 of ImmunediveRsity was used .
Donors, samples, demographic data, and sequencing metrics
Demographic data and clinical parameters
Number of individuals, n
Male, n (%)
3 (30 %)
2 (28.6 %)
4 (44.4 %)
1 (25 %)
Age in years, median (range)
Days after symptom onset, median (range)
Type of infection, n (%)
3 (30 %)
3 (42.9 %)
0 (0 %)
0 (0 %)
7 (70 %)
4 (57.1 %)
9 (100 %)
4 (100 %)
Serotype, n (%)
2 (20 %)
3 (33.3 %)
5 (50 %)
5 (55.6 %)
3 (30 %)
1 (11.1 %)
Hemoglobin, mean (SD)
Platelets, mean (SD)
Media ± SD
Media ± SD
Media ± SD
Media ± SD
73,197 ± 25,500
65,812 ± 23,151
66,082 ± 27,433
65,016 ± 47,798
Pass filters sequences
65,962 ± 23,636
56,459 ± 22,634
55,498 ± 25,926
56,754 ± 44,401
Heavy chain clonotypes (unique)
4961 ± 2590
2751 ± 858
5952 ± 4027
2536 ± 806
Heavy chains lineages (unique)
12,563 ± 5334
8672 ± 5687
14,337 ± 8620
7803 ± 2534
Estimation of the cellular composition and origin of sequencing reads
This scenario changes substantially in the post-convalescent phase, where the predominant IgG-expressing B cells are mB (average 95.8 %) [43, 44]. Under these conditions, sampling one read derived from an mB cell is common (p = 0.64) and its probability decreases below 0.012 until a lineage size threshold of 10 reads or higher is reached (Fig. 3c). The results of Monte Carlo simulation indicated that in patients with acute dengue, the majority of sequencing reads and the resulting lineages derived from ASCs. In contrast, during post-convalescence, lineages above 10 reads mainly derived from ASCs. However, we cannot exclude the possibility that in patients sampled at early-symptom onset, the proportion of ASCs may be similar to those sampled during the post-convalescent phase.
Differential usage of IGHV families and segments in acute phases
IGHV differential (ΔA − Pc) usage summary
Family or segment
The estimation of IGHV usage has limitations because it is based on a comparison between a predominantly DENV-specific plasmablast repertoire (Fig. 3b) , with the repertoire of a mixed IgG+ non-DENV-specific mB cell and plasmablast population during the post-convalescent phase (Fig. 3c). We estimated that during the post-convalescent phase, the probability of sampling an mB cell containing 30 reads or higher was very low (≤2.06447E − 06). To compare IGHV usage in plasmablasts during the acute and post-convalescent phases, we filtered out lineages below 30 reads, yielding similar results to those in the bulk analysis, but adding IGHV2-5 as significantly overrepresented (ANOVA p < 0.001) during the acute phase. Table 3 summarizes the analysis of differential IGHV family/segment usage in terms of the level of aggregation (lineages, clonotypes, relative expression, and filtering according to lineage size) and of differences between clinical and immune status. Because this analysis was performed using unsorted B cell populations, it is not possible to know the exact number of B cells involved. However, these results suggest a potential selection bias by DENV of B cells using segments of the IGHV1 family, particularly IGHV1-2, IGHV1-18, and IGHV1-69. Although IGHV1-2 raw expression was significantly increased in acute DWS+ (Additional file 7B; two-way ANOVA, p < 0.05), no differences were found at the clonotypic frequency level (Additional file 7C). Such differences in relative IGHV transcription (raw IGHV usage) may imply different proportions of cells with high IgG versus low IgG transcription, and not differences in the number of B cells using a particular IGHV segment. Biased usage of particular IGHV segments in response to a common pathogen in different individuals suggests that recognition is highly influenced by VH regions other than CDRH3 . Thus, the composition of such biased IGHV expansions should be polyclonal. Indeed, digital CDRH3 “spectra-typing” for biased IGHV segments at the lineage level confirmed this to be the case (Additional file 8).
To address if allelic variation in IGHV segment could influence the expansion of particular IGHV segments, we characterized the IGHV genotypes for IGHV1-2, IGHV1-18, and IGHV1-69 (Additional file 9). We found no correlation between allele type and expansion in the corresponding IGHV segments.
Lower SHM in the acute phase
In mice, marginal zone (MZ) B cell subsets are less dependent on T cell help, can be class-switched to IgG, have lower SHM rates, and have a differential IGHV usage , suggesting that in human DENV infection, the participation of a particular IgG+ B cell subset using unmutated or poorly mutated IGHV segments may take place. To determine if the reduction of SHM particularly affected certain IGHV segments during acute DENV infection, we calculated SHM rates according to IGHV segment. Significantly lower levels of SHM during acute DENV infection compared to post-convalescence were observed for IGHV1-2, IGHV1-18, and IGHV1-69 (p < 0.001) (Additional file 11). As for the total repertoire, significantly lower levels of SHM of IGHV1-2 were observed in acute DWS+ compared to acute DWS− (p < 0.001) and in acute secondary versus acute primary infections (Additional file 11A, B). In the case of IGHV1-18 and IGHV1-69, acute secondary infection had significantly lower SHM levels than acute primary infection (Additional file 11D–F); however, no significant differences were observed between acute DWS+ and DWS− (Additional file 11).
To avoid potential non-independence effects imposed by sampling clonally related lineages, we also performed the same SHM estimation but instead of random lineage subsampling in each library, we randomly subsampled 250 clonotypes and performed the SHM analysis in the corresponding largest lineage. The results using this approach agreed with the lower SHM rates in the bulk analysis, indicating that our SHM estimates were not the result of sampling bias (Additional file 12).
Moreover, to discard the possibility that clonotype (CDRH3) clustering threshold identity (97 %) could artificially sub-estimate SHM levels, we performed an analysis with the reconstructed repertoire at a CDRH3 threshold identity of 92 %. Under these parameters, SHM levels were equally low during the acute phase (Additional file 13).
As shown in Figs 5 and 6a, global SHM rates were lower in acute DENV infection, suggesting that this effect is not restricted to only IGHV1-2, IGHV1-18, and IGHV1-69. Calculation of SHM rates for all IGHV segments during acute and post-convalescent DENV infection and for controls (subjected to hierarchical clustering according to IGHV segment) revealed significantly higher SHM rates in controls and post-convalescent individuals than in patients with acute DENV infection (Mann–Whitney U test, p < 0.001) (Additional file 14).
Because the number of lineages belonging to a given B cell clonotype is the result of SHM, a straightforward prediction derived from lower SHM rates is that the number of lineages per heavy chain clonotype during the acute phase of DENV infection will also be low. Consistent with our observations, the clonotype per lineage ratio (1/lineages) was significantly reduced during the acute phase of DENV infection in both DWS+ and DWS− (Kruskal–Wallis test, Dunn’s correction, p < 0.001).
Convergent CDRH3 signatures in acute dengue infection
We also searched for convergent CDRH3s regardless of their presence in the dataset described in  (de novo). We found 1365 clonotypes representing 269 identical CDRH3s shared in at least three individuals during acute infection (0.9 % of all clonotypes in acute infection). Interestingly, among shared clonotypes there was a predominant CDRH3 length of 10 residues (70 %) (Fig. 8b–e). Two CDRH3s, ARQFGNWFDS and ARQWGNWFDL, were shared in 10 individuals (10/19, 52 % of individuals) (Fig. 8e). A CDRH3, ARQLGNWFDS, present in nine individuals was similar, although not identical to, the ARQIGNWFDP signature described in  (differences in italics). Finally, we addressed whether the convergent heavy chain clonotypes were less hypermutated. We sampled the largest lineage of convergent and non-convergent VH clonotypes as in Additional file 12. As expected, lower SHM rates were found in convergent VH lineages (Fig. 8f).
Understanding of the immune response to DENV infection has been hampered by the lack of adequate experimental models , but represents an urgent goal for developing safe vaccination strategies and as the basis for understanding the role of population immunity in disease transmission dynamics . Using an IgG Rep-Seq approach, we have shown that in the course of an acute DENV infection there is an increase in B cell clonal diversity in peripheral blood, presumably as a result of DENV-specific plasmablast mobilization. We also documented differences in the relative frequencies of B cells using certain IGHV segments belonging to the IGHV1 family according to clinical status. More importantly, we found a paradoxically low SHM frequency in the acute phase, as compared to the corresponding post-convalescent phase. Also paradoxical is the fact that the SHM frequency was even lower in secondary than in primary DENV infections. Interestingly, for some IGHV segments such as IGHV1-2, the SHM rates were lower in DWS+ compared to DWS− infection.
HTS of the B cell repertoire has been applied to explore higher order structural properties of the antibody repertoire [35, 51], track leukemia residual disease , uncover clonal lineages and patterns of SHM profiles in broadly neutralizing antibodies in human immunodeficiency virus , as well as to analyze the antibody response in influenza virus infection  and vaccination [23, 55, 56]. It is particularly relevant for the work presented here to explore convergent antibody signatures in DENV infection . Being a relatively recently developed approach, there are substantial methodological and analytical differences by which the research groups have coped with the challenge of mining the lymphocyte repertoire complexity. An important methodological difference is whether the starting material for Ig sequencing is DNA or RNA. Sequencing DNA has the advantage of providing a single copy per B cell as template for PCR amplification, thus, assuming unbiased PCR amplification, the clonal size is a reflection of the number of clonally related B cells in a given clone. An important advantage of using RNA-derived libraries for sequencing, particularly those generated by 5′RACE-PCR, is that less PCR amplification bias is expected owing to the use of a single primer pair based on invariant amplicon flanks . However, in contrast to DNA sequencing, differences in B-cell receptor (BCR) expression related to differentiation stage hamper an accurate estimation of clonal composition and size. To cope with potential distortions of Ig expression levels derived from unsorted B cells, we collapsed sequencing reads according to common clonal origin (heavy chain clonotypes) or further into lineages (consensus of sequences from a common clonal origin displaying a differential SHM pattern). Using this approach, IGHV frequency usage and SHM levels were quantified with respect to the corresponding denominator, reducing biases related to Ig expression levels.
In the context of DENV infection, we used the absolute lineage number, rarefaction analysis, and Shannon–Weaver index (entropy) as an approximation to estimate diversity in a subsample B cell repertoire (Fig. 2, Additional file 6). These analyses suggest that during acute DENV infection there is an increase in B cell diversity. An increase in B cell clonality due to clonal expansion using P[collision] was recently described . We argue that our results are not contradictory to this; P[collision] is estimated by replicate sampling and measures the probability of finding clonally related B cells in the replicates. Thus, clonal expansions can be readily assessed and proved during the acute phase of DENV infection . Here, because we used RNA as the starting material and sequenced a unique sample, we cannot evaluate clonal expansion directly (because clonally related sequences may be clustered during the reconstruction of clonotypes and lineages). Nevertheless, we interpret the higher species richness in rarefaction analysis and the increased Shannon–Weaver index as the result of higher numbers of circulating B cells, which could be explained, at least partially, by the massive plasmablast migration that occurs during the acute phase of the disease [15, 19, 42, 57].
Preferential IGHV usage has been described in antigen-specific B cells in models of cytopathic viral infection. In vesicular stomatitis virus (VSV) infection in mice, a rapid initial IgG-neutralizing response of defined germline unmutated IGHV segments (VHQ52) confer protection. Secondary challenge is associated with a shift in the use of other segments (VH7183 and VHJ558) and the occurrence of SHM [58, 59].
The generation of unmutated IgG+-switched mB cells beyond the VSV model has been described in a model of Polyomavirus infection  in Bcl6-deficient mice, which are devoid of TFH cells and thus lack a GC reaction [61, 62]. Also in mice, an extrafollicular response to Salmonella with sufficiently low levels of SHM to promote affinity maturation was recently described . Strikingly similar results have been obtained in human Rotavirus infection, where the response to VP6 is mediated in part by class-switched mB cells exhibiting low hypermutation rates that predominantly use IGHV1-46 . These observations have been generalized as part of the concept of “natural antibodies,” which can be IgM, IgG, and IgA. These are encoded by unmutated germline antibody genes, are produced as a rapid T cell-independent response against a variety of viruses with relatively high affinity, and recognize repetitive structures such as viral capsids, but are intrinsically poly-reactive . A notable example of this type are IGHV1-69-coded antibodies that have been recurrently implicated in recognition against influenza A, hepatitis C, and human immunodeficiency viruses [45, 48, 66–70].
Interestingly, lower IgG SHM rates were found in acute disease, and appeared to be more accentuated in DWS+ than in DWS−, and in secondary more than in primary acute infections. This effect was particularly clear in IGHV1-2-expressing IgG B cells. Consistent with its role in natural antibody recognition, IGHV1-2 is frequently used by neonatal cord blood IgM+ lymphocytes  and in MZ B cell lymphomas [73, 74], which are thought to derive from chronic pathogen-mediated selection . MZ B cells are capable of mounting class-switched and class-unswitched T-independent as well as T-dependent B cell responses . Normal MZ B cells produce rapid responses to blood-borne pathogens and could be the source of natural IgM and IgG responses against DENV. However, no modification in MZ B cells was observed in children with acute DENV infection . Moreover, human MZ B cells are usually hypermutated , thus suggesting that during acute DENV infection, MZ B cells are not the source of circulating poorly mutated IgG+ B cells.
Among other limitations of the HTS approach is that our VH libraries derived from total peripheral blood IgG-expressing B cells; as such, we have no information regarding the relative contribution of different B cell subsets or their antigen specificity. However, in support of our results, a preferential use of the IGHV1 family in plasmablasts, but not mB cells, has been described in acute DENV infection . Moreover, the high proportion of DENV-specific plasmablasts occurring during acute infection  provides further support to the claim that the observed repertoire differences may be DENV-specific as well.
Another limitation of working with unsorted IgG+ B cells, namely mB cells and ASCs (plasmablasts and plasma cells) is that their relative proportions vary dramatically during acute dengue infection, making it difficult to compare their respective repertoires. We used a Monte Carlo simulation method to estimate the probability of sampling either subpopulation depending on the relative proportions of mB cells and ASCs. A limitation of this simulation is that it cannot discriminate ASC from mB cells that have proliferated extensively. However, the lack of variation in the proportion of mB cells in acute dengue argues against this possibility [19, 57]. Interestingly, we estimated that the probability of sampling mB cells during acute dengue is negligible owing to the large number of plasmablasts and their corresponding high Ig expression levels. Likewise, during the post-convalescence phase, the probability of sampling an mB cell larger than 30 reads is negligible as well. This approach allowed us to partition the repertoire according to Ig expression level and to confirm our observations regarding IGHV usage and SHM level differences during acute disease. These experiments also highlight an additional advantage of using 5′RACE-PCR for Rep-Seq.
We are beginning to elucidate the molecular basis for T-independent CSR to IgG. In mice, TLR7 and TLR9 synergize with BCR signaling to promote activation-induced cytidine deaminase expression, which is required for CSR and SHM . Although both processes are functionally coupled, it is not clear how SHM is prevented in B cells undergoing T-independent CSR. TLR-mediated signaling pathways are implicated in promoting T-independent CSR  and T-independent IgG responses against Polyomavirus require MyD88 . Endosomal DENV recognition by TLR7 could provide a synergic signal with the BCR for T-independent CSR anti-DENV cross-reactive B cells (Fig. 9).
The proposed model of extra-follicular B cell responses offers a potential explanation for the production of low-affinity sub-neutralizing and cross-reactive IgG antibodies that may promote disease enhancement. Thus, in the case of DENV vaccination and analogously to observations in Polyomavirus-immunized mice , strategies that promote T-independent B cell responses (i.e., TLR7 agonist) could be associated with a higher risk of severe disease upon reinfection than strategies that do not [78, 79].
Our results in B cell repertoire mining suggest that during acute dengue infection, in parallel with a GC-dependent pathway, a GC-independent effector differentiation pathway may occur, which manifests as preferential IGHV gene use and low SHM in IgG class-switched B cells. This non-GC pathway may not be restricted to primary infections, given that SHM levels were even lower in secondary acute infections and was more striking in acute DWS+. Further research is required to obtain definitive proof of a GC-independent B cell differentiation in response to dengue infection, as well as to define the role of poorly mutated IgG in terms of poly-reactivity and ADE. Nevertheless, our findings are relevant for understanding of the immune response to DENV and future vaccine development.
analysis of variance
B cell receptor
complementarity determining region heavy 3
class switch recombination
dengue without warning sings
dengue with warning sings
enzyme-linked immunosorbent assay
memory B cell
proportion of mutations in VH region
rapid amplification of cDNA ends
reverse transcriptase polymerase chain reaction
reporter virus particles
trivalent inactivated influenza vaccine
vesicular stomatitis virus
We would like to thank Mauricio Vazquez-Pichardo for support with DENV genotyping, and Marbella Ovilla-Muñoz and Helena Kuri for laboratory assistance. Special thanks to Scott D. Boyd and José Ramos-Castañeda for critically reviewing the first version of the present manuscript.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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