Figure 5
The p53-NF-κB complex is present at the GIS regulatory region in human dilated cardiomyopathic hearts. (a-c) Human left ventricular tissue sections immunostained for NF-κB/RELA showed that NF-κB/RELA (in brown) was predominantly cytoplasmic in control left ventricule (a) but nuclear in both myocytes (open arrows) and fibroblasts or non-myocytes (closed arrows) of cardiomyopathic left ventricule (b,c). (d) No primary antibody control. (e,f) Sections were also immunostained for both a marker of oxidative DNA damage (8-oxoG, in brown) and activated p53 (phospho-p53ser15 (e); phospho-p53ser20 (f); in black with closed arrows). (g) Myocytes were distinguished from non-myocytes and fibrotic tissue both by their characteristic striations and positive staining for ankyrin (in brown). Bar represents 100 μm. (h) Left ventricular tissues from normal hearts and cardiomyopathic hearts were used for p53-RELA re-ChIP. Results represent fold enrichment of real-time qPCR for GIS and are representative of two replicated experiments using the same eight left ventricular samples. **P < 0.0005. Patient details for these left ventricular samples are in Additional file 1.