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Table 6 Validation of DKK1, UGT1A and EEF1A1 overexpression in the resistant cells

From: Networking of differentially expressed genes in human cancer cells resistant to methotrexate

   Expression
Gene Cell line Microarray RT-PCR validation
DKK1 HT29 4.25 5.66 ± 0.23
  Caco-2 2.56 1.96 ± 0.03
UGT1A MCF-7 15.90 23.41 ± 0.94
  MDA-MB-468 19.27 16.28 ± 0.19
EEF1A1 MIA PaCa-2 2.29 3.89 ± 0.16
  K562 2.75 2.38 ± 0.47
  Saos-2 2.05 1.85 ± 0.15
  1. The expression levels for the three node genes (DKK1, UGT1A and EEF1A1) derived from both the microarray data (average of the overexpressed UGT1A family members) and real-time RT-PCR are given. All the results are expressed as fold changes relative to the sensitive cells and values are the mean of triplicate experiments ± SE.