Figure 7

Surface receptor expression on vaginal antigen-presenting cells. (a) Transcriptional levels of CD209 (DC-SIGN), OLR1 (LOX-1), CLEC10A (DC-ASGPR) and CLEC4A (DCIR) in the four vaginal APC populations. Bar charts represent the mean ± standard deviation of batch-corrected expression data. (b) Fluorescence-activated cell sorting (FACS) analysis of CD209, DC-ASGPR, LOX-1, DCIR, DEC-205 and CD40 expression on the surface of vaginal APC subsets. Vaginal cell suspensions were stained with the indicated antibodies and gated as described in Figure 1b. Gray histograms represent isotype controls. Data are representative of 10 donors. (c) Frozen tissue sections were stained for (i) DC-SIGN, DC-ASGPR or LOX-1 (green), CD1c (light blue); and CD14 (red); or (ii) DCIR (red), CD1c (light blue) and CD14 (green); or (iii) DEC-205 (green), CD1c (light blue) and CD207 (red) (DAPI (dark blue), ×20, the horizontal bar represents 100 μm). Data are representative of four to eight independent experiments (left panel) or combined (right panel). Isotype controls are presented in Additional file 18.