Figure 4

In vivo parasite gene expression clusters by patient. (A) Hierarchical clustering of expression of 328 genes measured by nCounter® from 13 tissue samples collected at autopsy, collected from five patients and three organs. Clustering demonstrates that samples cluster by patient, rather than by organ, suggesting that parasite physiology within a patient is conserved. (B) Hierarchical clustering of global expression profiles imputed from nCounter® (n = 3,696 genes) shows that within-patient clusters remain intact. Note that two outliers in the first analysis (P1, Brain and P2, Heart) are further delineated as true outliers after imputation.