Figure 4

Detection of mutations by RNA-seq. (A) Eighteen genes with at least one mutation (out of 90 genes screened) across the 49 population primary breast tumors are shown, in order of frequency (see totals and percentages to the right of each gene row). Mutant allele frequency is indicated by the box color (see key below matrix). All mutations are non-synonymous missense mutations except those indicated by F (frameshift) and X (nonsense). Tumor sample dendrogram is as in Figure 3A. Predicted mutant amino acids are shown for (B) PIK3CA which encodes the p110-alpha catalytic subunit of the phosphatidylinositol-4,5-bisphosphate 3-kinase oncogene, and (C) TP53 which encodes the tumor suppressor TP53.