Fig. 2
From: Functional fingerprinting of human mesenchymal stem cells using high-throughput RNAi screening
High-throughput screening in MSCs produces robust results between different primary cell preparations. a Relative distribution of positive UBC (red) and negative Rluc (blue) controls used in the kinome-wide screen based on their deviation from the screen mean (z-scores). Technical replicates from the same MSC donor are shown, both displaying the high dynamic range of viability effects detectable by the screen. b Probability plot of the screening results, comparing theoretical quantiles assuming normal distribution (horizontal axis) against actual results of one representative high-throughput screen (vertical axis). Values are plotted according to their calculated z-score. In the low end of the distribution screening results diverge from the linear pattern, indicating biologically significant changes in cell viability. c Correlation plots of z-scores between technical replicates of the same MSC preparation (MSC1A and MSC1A), two MSC preparations from the same donor (MSC1A and MSC1B), and MSCs from two different donors (MSC1 and MSC2) show high correlation between MSC preparations (Pearson correlation is indicated)