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Table 2 Summary of predicted off-target sites for sgRNA_U1 and sgRNA_D1, and off-target variations identified

From: Correction of the auditory phenotype in C57BL/6N mice via CRISPR/Cas9-mediated homology directed repair

Predicted off-target sites

With two mismatches

With three mismatches

With four mismatches

Number of off-target SNVs and indels

Exonic

0

0

8

0

Intergenic

0

6

122

0

Intronic

2

6

82

0

  1. Summary of the predicted off-target sites with four or fewer mismatches for both sgRNA_U1 (total of 55 sites) and sgRNA_D1 (total of 173 sites) separated into genic type. Whole genome sequence analysis of the founder F0 mouse, used to establish the line that was phenotyped in this study, demonstrated no modifications to be present at these 228 predicted off-target sites
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Genome Medicine

ISSN: 1756-994X

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