Fig. 4

Preferential IGHV usage during acute DENV infection. a Heat map of hierarchical clustering of the difference between acute minus post-convalescent lineage frequency (ΔA − Pc) per IGHV family. Overrepresentation of IGHV family usage during acute phase is indicated in yellow tones, whereas blue tones indicate underrepresentation. Upper rows classify patients according to clinical status (DWS− green and DWS+ blue) and immune status (primary orange and secondary purple) (two-way ANOVA, Bonferroni correction for multiple testing, *p < 0.05). b Difference between acute minus post-convalescent (ΔA − Pc) according to IGHV segment, clinical status, and immune status. IGHV1-2 and IGHV1-69 are overrepresented in acute DWS+ (columns, DWS− green and DWS+ blue) and immune status (primary orange and secondary purple). A higher frequency of lineages using IGHV1-18 and IGHV1-69 in DWS+ is shown in red tones. DWS− dengue without warning signs; DWS+ dengue with warning signs; NA not applicable