Fig. 5

IgG B cell repertoire during acute DENV infection is associated with low somatic hypermutation (SHM) rates. SHM rates are shown according to clinical status (a) and according to immune status (b), measured as the proportion of mutations along the VH region [pM-VH (%)]. a The global SHM rate during the acute phase is significantly lower in acute DENV infection, but not as a result of the 2008–2009 seasonal trivalent inactivated influenza vaccination (TVI). b Global SHM rates are lower in the acute phase of DENV infection and significantly lower in acute secondary infection than in acute primary infection. Dengue without warning signs (DWS−) acute (A), green; DWS− post-convalescence (Pc), dark green; dengue with warning signs (DWS+) A, blue; DWS+ Pc, dark blue; d0 TVI, pink; d7 TVI, red; primary infection A, orange; primary infection Pc, brown; secondary infection A, light purple; secondary infection Pc, dark purple (Kruskal–Wallis test, Dunn’s correction for multiple testing, **p < 0.01, ***p < 0.001)